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separated as consequences of partial T cell signaling.
Weismann M. Guse AH. Sorokin L. Broker B. Frieser M. Hallmann R.
Mayr GW.
Integrin-mediated intracellular Ca2+ signaling in Jurkat T lymphocytes.
Journal of Immunology. 158(4):1618-27, 1997 Feb 15.
T lymphocytes interact with components of the extracellular matrix after
transendothelial migration on their way to sites of inflammation. To
characterize the molecular basis of the interaction between T lymphocytes
with different extracellular matrix proteins, we investigated the role of
intracellular Ca2+ as a signal mediating such interactions and identified
the cell surface integrins involved in this process. When Jurkat T
lymphocytes loaded with the calcium-sensitive fluorescent dye fura-2 were
placed on coverslips coated with human fibronectin, human collagen types
I, IV, and VI, human tenascin, human laminin I, or mouse laminin I, an
elevation in intracellular Ca2+ concentration was observed. In contrast,
contact of the Jurkat T lymphocytes with vitronectin and thrombospondin
did not induce Ca2+ signals in more cells as compared with control
measurements in which cells were in contact with only BSA or polylysine.
Furthermore, the percentage of Jurkat T lymphocytes responding with Ca2+
signals to collagen types I and IV, fibronectin, and laminin I was
completely reduced to levels observed on BSA or polylysine when the cells
were pretreated with specific anti-integrin Abs, suggesting a role for
cell surface integrins as mediators of cell matrix-induced intracellular
Ca2+ signaling. Similar results were obtained with peripheral human T
lymphocytes activated by phytohemagglutinin.
Реферат опубликован: 18/04/2005 (6263 прочтено)